A comparison of dual-enzyme immobilization by magnetic nanoparticles and magnetic enzyme aggregates for cascade enzyme reactions

Immobilized enzymes have been widely used for their efficiency, economy, and environment friendly. In this case, a simpler and effective immobilization method has been developed. In this study, the enzyme immobilized by magnetic beads with genipin (MGE) was prepared by mixing genipin-modified nano-Fe 3 O 4 with ( R )-1-phenylethanol dehydrogenase (( R )-PEDH) and recombinant glucose dehydrogenase (rGDH), and the magnetic cross-linked enzyme aggregates (MCLEAs) was prepared by stirring a mixture of the sulfur precipitate of the ( R )-PEDH and rGDH, poly-L-lysine, and cross-linkers. When o-phthalaldehyde (o-PA) was used as a cross-linking agent for the MCLEAs preparation, a protein loading of 99.9% and 62.93% recovery activity of ( R )-PEDH and 70.12% of rGDH were obtained. Compared to the MGE, the MCLEAs possessed better enzyme activity recovery. Furthermore, this study tried the synthesis of ( R )-1-(furan-2-yl) ethanol by the immobilized enzymes and obtained a concentration of 1.10   mM for the MCLEAs and 0.24   mM for the MGE at 48   h. This study explored a facile method for co-immobilization of ( R )-PEDH and rGDH to synthesize ( R )-1-(furan-2-yl) ethanol.