A turn-on anthraquinone-derived colorimetric and fluorometric dual-mode probe for highly selective Hg2+ determination and bioimaging in living organisms

Mercury ion (Hg 2+ ) is considered a harmful neurotoxin, and real-time monitoring of Hg 2+ concentrations in environmental and biological samples is critical. Fluorescent probes are a rapidly emerging visualization tool owing to their simple design and good selectivity. Herein, a novel fluorescence (FL) probe 2-(4-((6-((quinolin-8-yloxy)methyl)pyridin-2-yl)methyl)piperazin-1-yl)anthracene-9,10-dione ( QPPA ) is designed using piperazine as a linker between the anthraquinone group, which serves as a fluorophore, and N 4 O as the Hg 2+ ligand. The probe exhibits FL “turn-on” sensing of Hg 2+ because the complex inhibits the photo-induced electron transfer (PET) process. Moreover, QPPA can overcome the invasion by other possible cations, resulting in a clear color change from orange to colorless with the addition Hg 2+ . The chelation of QPPA with Hg 2+ in a 1:1 ratio. Subsequently, the theoretically determined binding sites of the ligand to Hg 2+ are validated through 1 H NMR titration. The in situ QPPA –Hg 2+ complex can be subjected to Hg 2+ extraction following the introduction of S 2− owing to its robust binding capacity. The exceptional limit of detection values for Hg 2+ and S 2− are obtained as 63.0 and 79.1 nM (S/N = 3), respectively. Moreover, QPPA can display bright red FL in the presence of Hg 2+ in different biological specimens such as HeLa cells, zebrafish, onion root tip tissues, and water flea Daphnia carinata , further providing an effective strategy for environmental monitoring and bioimaging of Hg 2+ in living organisms.