Biomimetic construction of multi-enzyme reactor through artificial antibody-antigen-directed immobilization for cascade catalytic conversion of cellulose to gluconic acid

To achieve the biotransformation of cellulose into gluconic acid, a multi-enzyme reactor comprising cellulase, glucose oxidase, and catalase was constructed through artificial antibody-antigen-directed immobilization. Catechol was used as a template to prepare artificial antibodies, which were then used to modify the enzymes through a Schiff base reaction to form artificial antigens. The artificial antibody and antigen were subsequently assembled via affinity recognition to immobilize the enzymes. The immobilization yields for all three enzymes exceeded 85 %, with specific activities of 43.48 ± 1.54 U/mg, 0.88 ± 0.06 U/mg, and 18.47 ± 1.43 U/mg, respectively. The immobilized enzymes demonstrated excellent adaptability, storage stability, reusability, and regenerability. Through the modular assembly of artificial antigen-antibody, the multi-enzyme reactor facilitated the cascade conversion of cellulose to gluconic acid.