Continuous production of aromatic esters from Chinese liquor yellow water by immobilized lipase in a packed-bed column bioreactor

Yellow water is a kind of Chinese liquor-making wastewater, and contains rich organic components such as alcohols and organic acids , if not treated, it can cause environmental pollution. Herein, continuous aromatic ester production from Chinese liquor yellow water by a packed-bed   column   bioreactor with   immobilized lipase was achieved. Inspired by interface activation of lipase, lipase from Aspergillus oryzae (AOL) was immobilized on LX-1000HA resin with silicone DC1–2577 modification (SH-LX-1000HA) and oleic acid modification (LX-1000HA-NH 2 -OA), respectively. Benefiting from the hydrophobic properties of the surface, the resultant AOL@SH LX-1000HA and AOL@LX-1000HA-NH 2 -OA were 2.3-times and 1.5-times higher activity than that of free AOL, reached 240 U/g and 156 U/g respectively. Meanwhile, the AOL@SH LX-1000HA and AOL@LX-1000HA-NH 2 -OA exhibited higher thermal stability , storage stability, and recyclability than free lipase. Furthermore, the aromatic ester production from yellow water by an packed-bed   column   bioreactor increased from 1.293 g/L to 3.976 g/L. This efficient continuous bioprocess presents great industrial potential for high value utilization from Chinese liquor yellow water.