Dual signal output detection of acetamiprid residues in medicine and food homology products via nanopore biosensor

Acetamiprid (ACE) is used in plant cultivation and their accumulation is a threat to ecology and health. Herein, a fluorescence/ion current dual-readout nanopore biosensor was designed for ACE detection. The carboxyfluorescein (FAM) labeled ACE aptamer was hybridized with complementary DNA (cDNA) to form partially hybridized double stranded DNA (dsDNA), and modified on the nanopore surface. ACE could specifically interact with FAM-aptamer and compete it against the nanopore surface. The probe changed from dsDNA to single-stranded DNA (ssDNA), which triggering the ionic current signal switched from “closed” to “open” and the fluorescence signal changed from “on” to “off”. The sensor exhibited linear range 0.5–100 ng/mL and with detection limit of 0.12 ng/mL. Meanwhile, the sensor can be employed for the detection of ACE residues in medicine and food homology (MFH) products. More importantly, the dual signal output detection results can be cross-verified, ensuring the accuracy of the detection.