Effect of phase transition of soy protein-chitosan complexes on liposomes stability: The perspective of membrane structure and inlaid stabilization mechanism

The aim of this study was to construct soybean protein (SPI)-chitosan (CS)-liposomes (Lip) systems with different electrostatic strengths and to investigate the effects of the phase behavior and structural properties of SPI-CS on the structural homeostasis of Lip. It was demonstrated that the SPI-CS-Lips exhibited different aggregation behaviors due to the strength of the electrostatic interaction, which had an essential effect on the adsorption and stabilization of the Lips. The SPI-CS-Lips system was transparent at pH = 5 with a potential of 22.32 mV and an average particle size of 316.53 nm. At this time, the formation of more uniform polymers was also observed in the microscope. The stabilization of the SPI-CS-Lip system depended on the adsorption and fixed-point embedding of SPI-CS and Lip. From the membrane structural perspective, the lateral stacking efficiency of the phospholipid molecules was increased in the SPI-CS-Lip system at pH = 5, with the most excellent stiffening behavior exhibited by the Lip. This study was conducted to provide theoretical support for the construction and mechanism exploration of the novel Nano-Lip system.