Effects of vitamin D3 on lifespan in Drosophila at different yeast levels

Deficiency of vitamin D3 has been found to be associated with an increased risk of developing some common diseases and death. However, the exact effect of vitamin D3 intake along with a high-protein diet and its optimal intake is unclear. The purpose of this work is to evaluate the beneficial effects of vitamin D3 on longevity using the Drosophila model. Vitamin D3 did extend lifespan on a high-protein diet, but above a certain concentration, it had the opposite effect in males. Our data suggest that the effect of vitamin D3 on longevity is diet-dependent and concentration-dependent.