Enhanced Bioactive Compound Absorption on PMMA Microwell Plates via Fine Controlling Air Plasma Treatment Time for Disease Diagnosis Applications

Microwell plates absorb bioactive compounds and are commonly used for disease prediction, diagnosis, and monitoring. Chemical absorption is more effective than physical absorption for stabilizing these compounds. This study systematically investigates the fundamental mechanisms of air plasma-induced surface modifications in poly(methyl methacrylate) (PMMA), focusing on carboxyl group formation kinetics, morphological evolution, and optical property changes. Air plasma treatment enhances the hydrophilicity and surface roughness of the PMMA plates. Light transmission remains comparable to untreated plates for 10 min treatment durations. Treatment for 3 min significantly increases the large-molecular-weight carboxyl compounds, with minimal loss after wash buffer rinsing. Thus, a 3 min air plasma treatment optimally enhances PMMA microwell plates for effective bioactive compound absorption.