Fluorescent microsphere-based strip for sensitive and quantitative detection of etomidate and metomidate

In this research, we used a novel hapten to generate a sensitive monoclonal antibody (mAb) 2C3 that targeted etomidate (ET) and metomidate (MT). We used this mAb to establish a lateral-flow immunoassay (LFIA) that incorporated fluorescent microsphere sensors, enabling both the qualitative and quantitative detection of ET and MT within 10 min. Analysis indicated that the visual colorimetric values for ET and MT in water samples were 0.3 µg/kg, respectively, with quantitative detection ranges of 0.08 to 1.31 µg/kg and 0.08 to 2.21 µg/kg, respectively. The visual colorimetric values for ET and MT in urine samples were 0.3 to 1 µg/kg, with quantitative detection ranges of 0.12 to 3.13 µg/kg and 0.09 to 2.98, respectively. The visual colorimetric values for ET and MT in serum samples were 3 µg/kg, with quantitative detection ranges of 0.15 to 21.2 µg/kg and 0.08 to 13.8 µg/kg, respectively. Recovery tests and detection were conducted in water, urine and serum samples, validating the reliability of this method in real clinical samples. Collectively, our novel LFIA provides a promising option for rapid on-site detection of ET and MT.