Forsythin-Loaded PET Membrane with Variable Wettability to Construct a Double-Layer Dressing for Wound Healing

The urgent need for efficient wound dressing requires comfortability, reasonable humidity, and anti-inflammatory and pathogen anti-infectivity properties. Herein, the PET micronanofiber pads loaded with forsythin (FT) are successfully designed and prepared by electrospinning. The asymmetric wettability double-layer micronanofiber pad (PET@FT10%/PET) has been developed by utilizing the hydrophobic properties of PET. The addition of FT endows super hydrophobic PET with excellent hydrophilic properties and absorbent properties to ensure the dryness of the wound environment. Meanwhile, the hydrophobic PET layer avoids the adhesion of dust and bacteria, providing a barrier to prevent pollution of the complex external environment for the drug-carrying layer. Furthermore, the PET@FT10%/PET double-layer micronanofiber pad provides favorable conditions for wound healing with the validation by inhibition of representative pathogens Escherichia coli and Staphylococcus aureus, and the expression of pro-inflammatory factors (IL-6 and TNF-α), and the cell survival rate also achieved excellent results. Therefore, the PET@FT10%/PET double-layer micronanofiber pad designed in this paper provides a neoteric strategy with an asymmetric structure design to avoid contamination and accelerate drug delivery for wound healing.