Laminaria japonica polysaccharide nano‑silver film with synergistic antibiosis for wound healing

Facing the clinical challenge of uncontrolled bleeding and infection after trauma, it is urgent to obtain a new type of dressing with antibacterial and wound healing promoting activities and good biological safety. Polysaccharide was extracted from Laminaria japonica Aresch by water extraction and alcohol precipitation, and LA-1 was obtained after separation and purification by DEAE-52 cellulose column. LA-1 had →2,6)-β-Manp-(1→) as the main chain and →4)-OAC-β-Galp-(1→, →3)-α-Fucp(1→, →4)-β-ManpA-(1→ and →4). Then, A kind of (PSx%AN) membrane with hemostatic and antibacterial functions was prepared by solution casting with LA-1 as green reducing agent and stabilizer. The (PSx%AN) film was formed by the cross-linking reaction of LA-1, AgNO 3 , polyvinyl alcohol and starch (3:1), in which the starch interacted with the polyhydroxy structure of polyvinyl alcohol to form hydrogen bonds, which endowed the film with good mechanical strength and biocompatibility. In order to comprehensively evaluate the performance of (PSx%AN) membrane, the oxidation resistance, antibacterial activity and cytotoxicity were tested, and animal experiments were also carried out. The results of the anti-oxidation and anti-bacterial experiments showed that the (PSx%AN) film not only exhibited good anti-oxidation performance, but also demonstrated a strong inhibitory effect on S. aureus and E. coli , with the maximum inhibition zone diameters of 16.8 ± 0.3 mm and 11.0 ± 0.2 mm respectively. The full-thickness skin defect experiment demonstrated that the (PS1%AN) membrane exhibited superior results compared to other groups on the 11th day. Specifically, it effectively increased the hydroxyproline concentration to 4.69 ± 0.19 μg/mL and the total protein content to 551.09 ± 7.19 μg/mL. Additionally, it reduced inflammatory responses, shortened wound healing time in mice, and promoted tissue repair. These findings provide a novel perspective for the (PSx%AN) membrane as a potential hemostatic and anti-infective wound dressing.