Quinine and vancomycin bilayer modified chiral stationary phase for enantioseparation and mechanistic study

The achievement of universal and efficient enantioseparation by chiral stationary phase (CSP) has been considered as challenging research. In this work, a bilayer-modified chiral stationary phase (named Sil-QN-VAN) based on vancomycin and quinine was constructed first. The chiral recognition abilities of quinine and vancomycin were combined on a single column, achieving the synergistic effect for enantioseparation. Compared with monochiral columns, the Sil-QN-VAN column exhibited excellent enantioseparation capability and could be used for separating multiple chiral drugs. The chiral separation mechanism was studied using molecular simulation techniques. In addition, the effects of temperature and injection volume on chromatographic separation were investigated. This work provides ideas for the development of efficient and universal chiral stationary phase materials.