Solution-blow spinning halloysite nanotube loaded chitosan-polyethylene oxide fibrous membrane as hemostatic dressings

In this study, chitosan/polyethylene oxide fibrous membranes (CS/PEO) with varying halloysite nanotubes (HNTs) contents were prepared by solution-blow spinning. The HNTs-CS/PEO membranes with a 12 wt% HNTs content exhibited excellent hydrophilicity, air permeability, and flexibility. The in vitro hemostasis time of the 12 % HNTs-CS/PEO was 217 s, and it was observed to form a stable thrombus. Moreover, the antibacterial efficacy of 12 % HNTs-CS/PEO against Escherichia coli and Staphylococcus aureus was 90.05 % and 69.86 %, respectively. Furthermore, the in vitro cytotoxicity study demonstrated the nontoxic nature of the 12 % HNTs-CS/PEO. The solution-blow spinning HNTs-CS/PEO fibrous membrane can potentially prevent the risk of distal thrombosis caused by separating individual HNTs and offer the advantages of a simple preparation and application process. This study presents a novel first-aid hemostatic dressing and offers a novel approach to loading inorganic nanoparticles onto fibers.