Zwitterionic polymers grafting of metal organic framework encapsulated boronic acid carbon dots as antibiofouling fluorescent probe for baicalin monitoring

The sensitivity and accuracy of fluorescence probes for biological samples are affected by not only interfering molecule compounds but also the nonspecific adsorption of proteins and other macromolecules . Herein, fluorescence probe based on zwitterionic sulfobetaine methacrylate polymer (PSBMA) as an antibiofouling layer and amino boric acid carbon dots encapsulated in the metal-organic framework UiO-66-NH 2 (UiO-66-NH 2 /BN-CDs) as a target recognition site was designed for the detection of baicalin (BAI). Owing to the introduction of BN-CDs into UiO-66-NH 2 with high specific surface area , the prepared UiO-66-NH 2 /BN-CDs@PSBMA probe exhibited a high adsorption capacity of 78.9 mg g −1 , while presented fluorescence enhancing and superior fluorescence selectivity to BAI at excitation and emission wavelengths of 400 and 425 nm, respectively. Connecting PSBMA with good hydrophilicity to UiO-66-NH 2 , resulted in an anti-protein capacity of over 96.3 %, effectively inhibiting protein interference with the fluorescence signal. By virtue of its good antibiofouling and recognizing capacities, the fluorescence probe exhibited a satisfactory detection range of 10–80 nmol L −1 , with a fairly low detection limit of 0.0064 μmol L −1 . Using the method to detect BAI in Goji berry , Sophora and Yinhuang oral solution, demonstrating its potential for the accurate and quantitative detection of BAI in complex biological samples.