A wearable Kevlar-based glove biosensor integrating proline-modified UiO-66 and lipase for on-site detection of methyl parathion

To meet the demand for nondestructive detection, we developed a truly human-wearable glove biosensor, which can be applied for in-situ analysis of pesticides on crop surfaces during the picking process. Herein, the Burkholderia cepacia (BCL)@UiO-66/Pro functionalized Kevlar-based laser-induced graphene (LIG) three-electrode glove sensing system was equipped with biocompatible semisolid electrolyte. In this system, Kevlar-based LIG electrodes showed the lowest sheet resistance of 28.26 Ω/sq. and I D /I G ratio value of 0.163 with optimal laser power of 9 %. Importantly, the BCL@UiO-66/Pro modified on Kevlar-based LIG electrode enhanced the catalytic efficiency of the BCL, the absolute value of the redox potential increases from the −0.412 V to −0.664 V due to an increase in the immobilization efficiency of BCL on UiO-66 after proline modification. In addition, the Kevlar-based wearable glove biosensor exhibited high repeatability, selective and sensitive electrochemical sensing of MP at concentrations ranging from 5 to 40 μM with a low detection limit of 0.84 μM. During the 10-day storage stability test, the fabricated Kevlar-based LIG biosensor demonstrated good reusability and excellent stability for MP detection with retention of more than 96 % of its initial response. Such wearable Kevlar-based glove biosensor could be a transformative approach for the in-situ analysis of pesticide residue on crops, which could also promote future precision agriculture development.