Dual-scavenging Mn3O4-PEI nanoparticles targeting ROS and cfDNA for acute pancreatitis therapy

Acute pancreatitis (AP) is a common inflammatory disease that seriously threatens people's lives and health. Excessive reactive oxygen species (ROS) and cell-free DNA (cfDNA) are significant contributors to the advancement of AP. In this study, a nanoparticle with a dual function to clear ROS and cfDNA was developed for the treatment of acute pancreatitis. Cationic polyethyleneimine (PEI) was used to modify Mn 3 O 4 , resulting in the creation of Mn 3 O 4 -PEI nanoparticles, which exhibited strong binding to cfDNA and the ability to scavenge ROS. In vitro, Mn 3 O 4 -PEI showed significant inhibition of ROS and cfDNA-induced inflammation and demonstrated therapeutic effects in a model of acute pancreatitis induced by sodium taurocholate (STC). This research introduces a new nanomedicine approach for the treatment of AP.