Zingiber officinale Polysaccharide Silver Nanoparticles: A Study of Its Synthesis, Structure Elucidation, Antibacterial and Immunomodulatory Activities

Green-synthesized metal nanoparticles show promise in nanomedicine and material engineering. In this study, the polysaccharide of Zingiber officinale (ZOP) was used as a raw material. Through single-factor experiments and a response surface methodology, the optimum synthesis protocol of Zingiber officinale polysaccharide silver nanoparticles (ZOP-NPs-AgNPs) was determined as follows: V(AgNO 3 ):V(ZOP) = 2.98:1, 59.79 °C, 3 h, pH 9, and 20 mL NaCl, achieving a 92.51% silver chelation rate. Structure analysis revealed that ZOP-NPs-AgNPs were spherical or quasi-spherical, with a particle size < 20 nm and a face-centered cubic crystal structure, which has good thermal stability. Subsequent studies explored the antibacterial and immunomodulatory effects of ZOP-NPs-AgNPs. The minimum inhibitory concentration (MIC) of ZOP-NPs-AgNPs against Escherichia coli and Staphylococcus aureus was determined to be 0.5000 mg/mL and 0.0310 mg/mL, respectively, while the minimum bactericidal concentration (MBC) was 0.5000 mg/mL and 0.0310 mg/mL, respectively. Additionally, ZOP-NPs-AgNPs significantly enhance RAW264.7 cell proliferation and phagocytosis and boost IL−1β, IL−6, NO, and TNF-α production. This confirms that ZOP can act as a green reductant and stabilizer, offering a new method for green nano-silver synthesis. This provides a sustainable way to produce antibacterial products and functional foods, and offers useful references for eco-friendly nano-silver applications.