Pyrococcus furiosus Argonaute biosensing platform based on hemin@ZIF-90 nanozyme-modulated signal amplification for sensitive detection of Salmonella typhimurium

Foodborne pathogens have posed a great threat on food safety and public health, and thus the development of accurate and sensitive methods for the detection of foodborne pathogens is of great significance. In this study, a novel Pyrococcus furiosus Argonaute ( Pf Ago) biosensing platform based on metal-organic framework (MOF) nanozyme (namely AgoZyme) was developed for the detection of Salmonella typhimurium . Inspired by the catalytic characteristics of horseradish peroxidase, we synthesized a Hemin@ZIF-90 nanozyme with peroxidase-like activity by encapsulating hemin into a zeolitic imidazolate framework-90 through a self-assembly strategy. By integrating the specific recognition and cleavage capabilities of Pf Ago with the highly catalytic performance of Hemin@ZIF-90, the AgoZyme biosensor exhibited excellent detection sensitivity and specificity. This biosensor had a limit of detection of 1 CFU/mL in a linear range from 10 1 to 10 8  CFU/mL. This work provided a new insight for designing and developing novel biosensors for food safety detection.