摘要
Titanium and its alloys, widely used as orthopedic implants, frequently encounter clinical implantation failure due to insufficient antimicrobial and osteogenic activities. To address these problems, a bioactive multifunctional coating (named TNPTL/Sr@TET) was established on titanium surfaces in this study. Bone-like porous TiO 2 nanotubes (TNT) were first constructed via anodic oxidation, followed by bio-functionalization involving the loading of Sr 2+ ions (TNPTL/Sr) mediated by phase-transited lysozyme (PTL) and the covalently grafting of bone-targeted tetracycline (TET) via the carbodiimide reaction. The morphology, chemical constitution, and surface wettability of the prepared coatings were thoroughly characterized via Scanning Electron Microscopy, Energy Dispersive Spectroscopy, X-ray Photoelectron Spectroscopy, Attenuated Total Reflection-Fourier Transform Infrared Spectroscopy, and water contact angle measurement. The results showed that regular TiO 2 nanotube arrays were developed with an average inner diameter of about 52 nm and a wall thickness of about 16 nm. Analysis of characteristic elements and functional groups confirmed that the amyloid-like PTL layer, Sr 2+ ions, and TET were effectively immobilized on the nanotubes in turn. The TNPTL/Sr@TET coating reduced the inner diameter, increased the wall thickness and roughness of nanotubes, and significantly improved the hydrophilicity of titanium surfaces. The TNPTL/Sr@TET coating accelerated in-situ deposition of cauliflower-like apatite, promoted early adhesion and proliferation of osteoblasts, and upregulated alkaline phosphatase, osteocalcin, and runt-related specific transcription factor-2. The TNPTL/Sr@TET coating also demonstrated strong antibacterial activity against S. aureus and E. coli . The TNPTL/Sr@TET coating presented here provides an innovative strategy to enhance both the osteogenic and antibacterial properties of titanium implants.
危险品化学品经营许可证（带存储）