A customized enzyme-like FeMOF@PtNPs aptasensor for rapid and sensitive colorimetric detection of aflatoxin B1 in feed

In this study, iron-based metal-organic framework@platinum nanoparticles (FeMOF@PtNPs) with outstanding peroxidase-like catalytic activity was synthesized using a simple one-pot hydrothermal method. Leveraging this catalytic platform, a cost-effective colorimetric aptasensor was developed for the detection of aflatoxin B1 (AFB1) residues in feed. The sensor exhibited a linear response to AFB1 concentrations ranging from 10 to 1000 nM, with a detection limit as low as 2.18 nM under optimized conditions. Furthermore, the sensor demonstrated excellent selectivity and stability. The reliability of the proposed aptasensor was validated by testing real feed samples, with results closely matching those obtained using the standard ELISA method. This suggests that the constructed colorimetric aptasensor holds great potential for practical applications. In addition to AFB1 detection, the proposed sensing platform can be readily adapted for various analytes by replacing the corresponding aptamer. This offers broad applicability in food safety, environmental monitoring, and medical diagnostics.