Injectable Thermo-Sensitive Chitosan Hydrogel Loaded with α-Mangostin Promotes Wound Healing in Bacteria-Infected Mice

One of the main challenges in wound healing is bacterial infection, and the emergence of antibiotic resistant superbugs in recent years has exacerbated these concerns. In this study, we developed a chitosan/tannic acid/poloxamer 407/poloxamer 188 thermo-sensitive hydrogel (CTPP). The bioactive compound α-Mangostin (α-M) extracted from mangosteen fruit was incorporated into CTPP (CTPP@α-M). The results indicate that CTPP@α-M can slowly and continuously release α-M within 11.5 h. In addition, CTPP@α-M effectively inhibits bacterial proliferation, accelerates collagen deposition, reduces inflammatory response, and promotes rapid wound healing in the presence of wound infection. The biosafety experiment showed that the hydrogel had excellent biocompatibility. Overall, these findings suggest that CTPP@α-M has the potential to become a potential treatment for repairing infectious wounds. Graphical In this study, CTPP hydrogel and CTPP @ α - M drug carrying hydrogel were prepared by physical crosslinking method. CTPP shows excellent performance and can be converted to semi-solid gel at 37 ° C. CTPP @ α - M showed strong inhibitory effects on MRSA and accelerated wound healing, reducing the expression of pro-inflammatory cytokines. The data show that CTPP @ α - M is a highly effective antibacterial thermosensitive hydrogel with excellent performance.