Construction of fluorescent probe based on phenoxazine for the detection of N2H4 in environmental, biological and food samples

Hydrazine (N 2 H 4 ) is widely used in agrochemicals and pharmaceuticals, but unintentional releases of hydrazine can be transmitted through food, organisms and the environment, ultimately posing a serious hazard to human health. Consequently, the development of a rapid and sensitive analytical tool for the detection of N 2 H 4 is necessary. In this study, we present the synthesis and application of a novel molecular probe ( Probe PM ). The probe is characterised by its simple synthesis, high yield and a range of analytical properties including very high sensitivity (limit of detection as low as 17.1 nM) and a pronounced fluorescence spectral blue shift of 146 nm. These attributes have enabled the successful implementation of Probe PM in the qualitative and quantitative detection and visualization of hydrazine in diverse environmental and biological matrices, including water samples, soil samples, food products, and biological systems. The probe has great potential for detecting environmental and food safety.