3D nanofiber sponge based on natural insect quaternized chitosan/pullulan/citric acid for accelerating wound healing

Extensive traumatic injuries and difficult-to-heal wounds, induced by many circumstances, impose a significant social and economic burden on an annual basis. Thus, innovative wound dressings that encourage wound healing are greatly needed. In this work, we prepared a novel insect chitosan (MCS) using waste pupal shells from housefly ( Musca domestica L.) culture. After conducting comparative investigations with commercially available chitosan, it was shown that MCS exhibited comparable qualities and may be used as a substitute source of commercial chitosan. A quaternized chitosan/pullulan/citric acid three-dimensional nanofiber sponge (3D-NS) of natural origin was prepared by electrostatic spinning and gas foaming techniques after MCS was quaternized. In vitro , tests showed that the 3D-NS had a higher liquid absorption capacity than the two-dimensional nanofibrous membrane (2D-NM). Additionally, the 3D-NS showed improved hemostatic, pro-cell proliferation, antibacterial, and anti-inflammatory qualities. In vitro , tests demonstrated that 3D-NS could inhibit the release of inflammatory factors, promote angiogenesis, accelerate collagen deposition, and promote wound contraction. These effects considerably facilitated the healing process of wounds in rats with full-thickness skin damage. In conclusion, the great bioactivity and physicochemical properties of 3D-NS render it an optimal candidate for developing novel wound dressings.