Construction of an electrochemical aptasensor based on a covalent organic framework for rapid and ultrasensitive detection of staphylococcus aureus

A chemically stable crystalline covalent organic framework (TpPa-1-COF) was successfully synthesized using simple mechanical ball milling method. Owing to its hydrophobic interaction, hierarchical pores, extended π-conjugation skeleton and excellent stability, the TpPa-1-COF can efficiently immobilize aptamer to fabricate an electrochemical sensor for detection of Staphylococcus aureus ( S. aureus ), achieving a low limit of detection of 0.28 CFU⋅mL -1 (3σ/S). Furthermore, the TpPa-1-COF-based electrochemical aptasensor exhibited good stability, excellent reproducibility and high selectivity. Notably, the TpPa-1-COF-based electrochemical aptasensor can quantitatively detect S. aureus in spike samples, demonstrating its potential contribution to food safety assurance.