Hydrothermally Synthesized Boletus Brucella-derived Carbon Quantum Dots as a Fluorescent Probe for the Detection of Vitamin B2

Background In the paper, Boletus Brucella was used as carbon source material to prepare carbon dots (CDs) by one-step hydrothermal method. The CDs had high quantum yield and high photostability. Methods A range of characterization studies were conducted on CDs, and the results showed that the average particle size of CDs was 5 nm, emitting blue fluorescence. The optimal excitation wavelength was 337 nm, and the emission wavelength was 440 nm. Results Based on the static quenching, the fluorescence of CDs could be effectively quenched by VB2. Therefore, a highly sensitive and selective fluorescent probe for detecting VB2 was constructed. The CDs were successfully used to detect tablets, human blood, and urine. Conclusion The recovery rate of VB2 was 97.55~99.45%, and the relative standard deviation was 1.29~3.76 (n=3).