Development and functional evaluation of curcumin-loaded zein-gum Arabic-flaxseed gum complex nanoparticles for anti-fatigue applications

In this study, zein-gum Arabic (GA)-flaxseed gum (FG) nanoparticles (ZGF) were prepared using the anti-solvent and electrostatic deposition methods to overcome the hydrophobicity and instability of curcumin (CUR). Additionally, the optimal mass ratio of GA to FG (4:1) and CUR to zein (1:40) was determined. Additionally, the initial concentration of the polysaccharide (0.04 %) was determined and the ZGF nanoparticles encapsulated with CUR (CUR-ZGF) were optimized. The formed CUR-ZGF nanoparticles were spherical, with a particle size of 188 nm and an embedding rate of 96.8 %. Furthermore, the CUR-ZGF nanoparticles showed excellent pH, thermal, storage, and salt stability. The encapsulate CUR exhibited antioxidant capacity and controlled release rate in the gastrointestinal digestive system in vitro. Animal experiments showed CUR-ZGF nanoparticles significantly enhanced exercise capacity and anti-fatigue effects. High-dose CUR-ZGF nanoparticles doubled exhaustion running time versus the model group, improving exercise endurance. Fatigue-related biochemical parameters (serum urea nitrogen, serum lactate, and creatine kinase) were significantly reduced, indicating rapid fatigue elimination. Meanwhile, significantly increased lactate dehydrogenase and fasting glucose levels suggested efficient energy replenishment to mitigate fatigue damage. These results indicate CUR-ZGF nanoparticles may be a promising natural anti-fatigue healthcare product in the future.