Rapid Screening of Lipase Inhibitors From Chrysanthemum Based on Ionic Liquid/Chitosan Bifunctionalized Magnetic Multi-Walled Carbon Nanotubes Immobilized Lipase

In this study, a novel ionic liquid/chitosan bifunctionalized magnetic multi-walled carbon nanotubes composite material (m-MWCNTs@CS@IL) was utilized for the first time to immobilize lipase. The immobilized lipase exhibited exceptional stability and reusability, as evidenced by its characteristic properties. A ligand fishing approach utilizing the immobilized lipase was developed to enable rapid screening of lipase inhibitors from Chrysanthemum . Three ligands were successfully screened from Chrysanthemum and then identified as cynaroside, quercitrin, and linarin by ultra-high performance liquid chromatography-tandem mass spectrometry. The IC 50 values of these three ligands were 76.77 ± 0.36, 83.01 ± 0.46, and 43.61 ± 0.77 µM, respectively. Furthermore, molecular docking analysis further confirmed the binding of three ligands to specific amino acid residues within the active site of lipase. This work presents a fast and efficient approach for screening lipase inhibitors from intricate natural sources, demonstrating promising prospects in discovering anti-obesity compounds.