Construction of a highly sensitive recoverable lipase@sodium alginate/cyclodextrin/graphene oxide film for triglyceride detection in dairy products

Triglycerides (TGA) are important clinical indicator for fatty liver, heart disease, hypertension and other endocrine disorders. Therefore, an accurate and stable TGA detector is crucial for a healthy diet. In this work, we developed a lipase-immobilized electrochemical biosensor with a novel conductive film for efficient detection of TGA. The lipase was embedded in sodium alginate (SA), cyclodextrin (CD) and graphene oxide (GO) film. Cyclodextrin had the effect of enhancing the pore structure of the films and had good binding ability with TGA, thus lipase@GO/SA/CD film showed good catalytic activity. The linear range of the TGA electrode was 0–7.5 mM. In addition, the detection limit was as low as 0.05 mM for the direct detection of TGA. The sensor was successfully recycled with average recovery values ranging from 90.6 % to 96.0 %. The lipase@GO/SA/CD film was suitable for TGA detection in dairy products detection, and provide a new sensing TGA platform.