Revolutionizing Escherichia coli detection in real samples with digital SERS aptamer sensor technology

Aptamer sensors based on surface-enhanced Raman scattering (SERS) technology have demonstrated great potential in the ultrasensitive and rapid detection of Escherichia coli ( E. coli ). Herein, this paper presents a digital SERS aptamer sensor. This sensor integrates ordered nanoscale array synthesis technology and digital analysis technology, enabling highly sensitive and rapid bacterial quantification. The ordered monolayer gold nanosphere arrays (Au NS) can form uniform and dense “hot spots” on the silicon wafer due to their uniform spherical structures and narrow gaps. Moreover, digital SERS is adopted to further optimize the signal uniformity so as to achieve precise quantification. The sensor modules are combined together through base pairing. The aptamers labeled with Raman tags are detached from the complementary DNA due to the competition of the target substance, thus realizing the detection of E. coli . The digital SERS aptamer sensor has been verified to possess excellent selectivity and reproducibility. It has a wide dynamic linear detection range from 1.0 * 10 1 to 1.0 * 10 9  CFU/ml and a detection limit of 0.657 CFU/ml, maintaining excellent specificity even in the presence of mixed bacterial interference. The spiked recoveries in actual samples range from 98.80 % to 99.81 %. Leveraging different aptamers and digital analysis, the sensor holds promise for food safety and environmental monitoring applications.