Multifunctional magnetic MOF under cover of erythrocytes for ROS scavenging and magnetic hyperthermia thrombolysis

The inadequacy of current antithrombotic therapies lies in the limited efficacy of pharmacotherapy and the poor targetability of drugs to thrombotic sites. To address these challenges, we developed a multifunctional nanoplatform, Fe 3 O 4 @MC-DEA-PBAP (FMDP), which integrates magnetic responsiveness and erythrocyte targeting for magnetic hyperthermia thrombolysis (MHT). FMDP is constructed using metal–organic frameworks (MOFs) crosslinking CaCO 3 /cyclodextrin as the base carrier, modified with 2-(Diethylamino)ethyl methacrylate (DEA) and 4-hydroxyphenylboronic acid pinacol ester (PBAP), then conjugated with nano-ferric oxide for MHT. DEA and PBAP make FMDP to adhere onto erythrocytes and scavenge reactive oxygen species (ROS), respectively. In vitro experiments proved that FMDP exhibits high photothermal conversion efficiency and robust magnetic responsiveness, enabling the thrombolytic efficiency of FMDP was three times that of uPA group. In addition, FMDP adhere to erythrocytes (83.5 %) and reach the thrombi under the cover of erythrocytes for MHT. In addition, FMDP effectively scavenges ROS, mitigating endothelial damages and promoting vascular regeneration. Overall, FMDP represents a promising therapeutic strategy for the comprehensive management of thrombosis, offering improved efficacy and reduced bleeding risks compared to conventional thrombolytic therapies.