Enzymatic preparation of structured phospholipids from soybean phosphatidylcholine and investigation of their phase behavior

The present study aimed to investigate the application of lysophospholipids from enzymatic degumming products and different acyl donors to form structured soybean phospholipids in the lipase-mediated acidolysis reaction. Sn -1 and sn -2 fatty acid compositions of structured phospholipids from soybean phosphatidylcholine (SP-SPC) were examined, indicating that the relative content of saturated fatty acid in SP-SPC sn -2 fatty acids increased. Liquid chromatography-mass spectrometry analysis suggested that there were seven SP-SPC molecules. Spectroscopy analysis and differential scanning calorimetry thermogram analysis indicated that the structure of SP-SPC was more stable than SPC due to its unique alkyl chain conformation and phase states. Polarized light microscopy observations suggested that SP-SPC had a strong birefringence phenomenon. The preparation of liposomes with SP-SPC showed a homogeneous double-layer with an almost constant C C strength. These results have important implications for the enzymatic preparation of structured soybean phosphatidylcholine and the information on its phase states.