Development of an activatable fluorescent probe for GGT activity detection in colitis diagnosis

Colitis is a common inflammatory bowel disease, and early diagnosis is crucial for effective treatment. Gamma-glutamyltransferase (GGT) has been suggested as a potential biomarker for colitis, but current detection methods face challenges in sensitivity and specificity. Therefore, developing a sensitive and practical tool, especially one that uses serum samples, is important for improving clinical diagnosis. In this study, a novel fluorescent probe was developed to detect GGT activity in serum samples. The probe was incubated with serum from both colitis and healthy mice, and fluorescence measurements revealed significantly higher signals in the colitis group, indicating elevated GGT activity. This demonstrates the probe's high sensitivity and its potential for identifying colitis through serum detection.The findings support the clinical use of this probe for detecting GGT activity in inflammatory diseases like colitis, offering a simple and efficient diagnostic tool for early diagnosis and treatment.