Green extraction of proteins and anti-skin aging peptides from Tricholoma Matsutake using natural deep eutectic solvents

Chronic oxidative stress and inflammation accelerate skin aging by inducing structural compromise in intracellular components and extracellular matrix (ECM) degradation. Plant-derived bioactive peptides have attracted great attention for their outstanding biological activities. However, before protein hydrolysis, the traditional extraction strategy of protein was unsustainable for its polluting solvents and reagents. Tricholoma matsutake ( T. matsutake ) is rich in proteins, vitamins, poly-peptides and double-chain polysaccharides. Several natural deep eutectic solvents (NaDESs) were synthesized for the efficient extraction of protein from T. matsutake . The highest yield of protein (52.5 ± 0.66%) was obtained from extraction at 35 of matrine/1,2-Propanediol (MaPd) concentration for 2 h, a solid-liquid ratio of 40 mL/mg. Furthermore, the optimal peptides were prepared by protein hydrolysis with 20% alkaline protease at 55 °C for 90 min. Notably, T. matsutake peptides were good in scavenging radicals and in decreased levels of IL-1α, IL-6. Meanwhile, T. matsutake peptides exhibited anti-skin aging ability by preventing the secretion of MMP-1 and MMP-3, degradation of collagen I and elastin. The study demonstrates that NaDES combined with enzymolysis extraction is a suitable, green technique with high potential of peptides from T. matsutake . Therefore, T. matsutake peptides can be considered for developing crude drug formulations and functional food or cosmetics materials with anti-inflammatory and Anti-aging properties.