Selenium nanoparticles modified with Bletilla striata polysaccharide, the antioxidant activity and the protective effects against ALI

In this work, Bletilla striata polysaccharide-modified SeNPs (BSPs-SeNPs) was prepared to address the instability of SeNPs as a result of its outstanding performance in the field of natural polymer materials. BSPs-SeNPs were synthesized using Na 2 SeO 3 as the selenium source and ascorbic acid as the reducing agent. The FTIR, XRD and EDS results demonstrated the successful synthesis of BSPs-SeNPs, which exhibited excellent nanoparticle characteristics, with a particle size of 66.9 ± 1.30 nm and Se concentration of 12.19 ± 0.62 %. Stability tests indicated that BSPs-SeNPs maintain structural integrity under various storage conditions, pH levels, temperatures, and ionic strengths. The mice acute liver injury (ALI) model was created with 300 mg/kg APAP, BSPs-SeNPs decreased serum ALT and AST values as expected. And the restored SOD, GSH and MDA values showed correlation with the in vitro antioxidant activity assay results (DPPH · , ABTS · , · O 2 − , and · OH). In general, BSPs-SeNPs is successfully synthesized, which enlightened the conjunction between natural polysaccharide and inorganic nanoparticles, but also encouraged the extensive use of BSPs in the field of pharmaceutical excipients.