Identifying Optimal Parts of Oriental Lily Bulbs for Large-Scale Propagation Using Tissue Culture Bulb Induction

Lily (Liliumspp.) is a perennial ornamental plant valued for its striking ornamental value and the edible and medicinal properties of its bulbs. Compared to other lilies, Oriental lilies are characterized by their large flowers and strong fragrance, making them one of the most popular types of lilies on the market. It is important to identify optimal parts of lily bulbs for rapid propagation of tissue culture seedlings. In this study, bulb scales of the Oriental lily ‘Pacific Ocean’ were used to explore the optimal parts for the tissue culture bulb induction. After 30 days of inoculation of lily scales in the induction medium, our results showed that Middle Central regions of the Inner layer scales (MCI) had the highest induction rate of 90.27 % among all regions from all layers of scales. In scales of all layers, the basal parts had the best longitudinal induction effects, followed by the middle and apical parts. Among all layers, the inner layers also had the lowest rates of contaminations. When taking both induction rate and contamination rate into account, MCI also represents the best choice among all compared parts. In summary, our results formed a practical guide for large-scale propagation of Oriental lily using tissue culture seedlings.