Biomimic oleuropein-loaded mesoporous polydopamine nanocomposite in injectable hydrogels inhibits cell apoptosis and antioxidant for myocardial infarction

This study addressed the fabrication of mesoporous polydopamine nanoparticles (MPD NPs) by an emulsion-induced interface assembly technique, followed by the incorporation of oleuropein (OLE) into MPD NPs via electrostatic attraction, resulting in OLE@MPD NPs. In the post-myocardial infarction (MI) context, these therapies were explicitly designed to eradicate reactive oxygen species (ROS) and promote MI healing. We additionally integrated them with a thermosensitive chitosan (CH) hydrogel (HGL) to generate an injectable composite hydrogel (OLE@MPD/CH HGL). Through in vitro tests, the OLE@MPD/CH HGL demonstrated remarkable ROS-scavenging capability in H9C2 cells subjected to oxidative stress and effectively inhibited their apoptosis. This study introduces an innovative method to enhance MI therapy by utilizing the OLE@MPD/CH HGL. This OLE@MPD/CH HGL efficiently diminishes localized oxidative stress caused by ROS and activates the HO-1/Nrf2 signaling pathway.