Wulff-type boronate acids-combined controlled explosive release nanocapsule for simultaneous detection of 2 kinds of bacteria

Foodborne pathogens pose serious risks to environmental protection and food safety. The low abundance of foodborne pathogens during the early stage of pollution makes the detection of them a challenge, and combining efficient bacterial enrichment with powerful signal release is a potential proposal. In this study, wulff-type boronate functionalized magnetic nanoparticles (MNPs) (B-N/APBA@MNPs) was designed, the binding pH and affinity of the original boronic acid were improved by teamed boronate affinity strategy. Characterization and capacity performance assessment ensure its successful synthesis and excellent capture performance for 2 bacteria. Meanwhile, controlled explosive release of carbon dots (CDs) nanocapsule was prepared by encapsulating CDs with characterized its successful synthesis and evaluate its fluorescence performance. The nanocapsule encapsulating strategy can effectively enhanced the fluorescent signal and avoided the interference of magnetic nanoparticles (MNPs) on CDs. Taking Listeria monocytogenes and Escherichia coli O157:H7 as a proof of concept, a dual-signal amplification platform based on Wulff-type boronate acids combined with controlled explosive release nanocapsule was established. Results showed an impressively low limit of detection (10 1  CFU/mL) and high recovery rates (84.95 % ∼ 116.27 %). Notably, the proposed dual-signal amplification strategy provides a design template for the detection of low concentration of bacteria in environmental detection and food safety.