NIR-Responsive Nano-Integrated Magnesium Phosphate Cement Promotes Bone Regeneration via Osteoblast-Exosome-Osteoclast Crosstalk

en This link goes to a English section zh This link goes to a Chinese section The limited osteogenic capacity of magnesium phosphate cement (MPC) has constrained its biomedical applications, underscoring the necessity to develop MPC with enhanced physical properties and bone-forming capabilities. In this study, a multifunctional MPC system was developed by incorporating zoledronic acid-loaded near-infrared (NIR)-responsive nanocarriers, strontium oxide (SrO), and hyaluronic acid (HA). The nanocarriers were constructed using dual-layer poly-dopamine (PDA) modification of mesoporous silica nanoparticles (MSNs), enabling controlled drug release and antibacterial efficacy under NIR stimulation. The optimized Sr-ZMP-HA MPC demonstrated prolonged setting time, near-neutral pH, superior injectability, and improved compressive strength. Immersion tests revealed its sustained degradation resistance. This composite material exhibited excellent biocompatibility along with enhanced osteogenic and angiogenic properties, particularly when activated by NIR irradiation. The experiments demonstrated that NIR-triggered Sr-ZMP-HA MPC promoted osteoblast-derived exosome secretion. These exosomes mediated miRNA transfer to osteoclasts, effectively suppressing their proliferation and differentiation while delaying bone tissue senescence. This dual-functional system, combining NIR-responsive nanomedicine with exosome-mediated intercellular communication, provided a novel strategy for developing advanced bone repair materials, potentially addressing current limitations in orthopedic applications through synergistic mechanical reinforcement and biological activation mechanisms. 摘要 en This link goes to a English section zh This link goes to a Chinese section 磷酸镁骨水泥(MPC)有限的成骨能力限制了其在生物医学中的应用,因此开发具有良好物理性能和骨形成能力的MPC是必要的。在本研究中,通过掺入唑来膦酸负载的近红外(NIR)响应纳米载体,氧化锶(SrO)和透明质酸(HA),我们开发了一种多功能MPC系统。纳米载体是通过介孔二氧化硅纳米粒子(MSN)的双层聚多巴胺(PDA)修饰构建的,能够在NIR刺激下控制药物释放和抗菌功效。优化的Sr-ZMP-HA-MPC表现出延长的固化时间,接近中性的pH值,优异的注射性和改善的抗压强度。浸泡试验表明其具有持续的抗降解性。这种复合材料表现出优异的生物相容性以及增强的成骨和血管生成特性,特别是当通过NIR照射时更加明显。实验表明,NIR触发的Sr-ZMP-HA-MPC促进成骨细胞衍生的外泌体分泌。这些外泌体介导miRNA转移至破骨细胞,有效抑制其增殖和分化,同时延缓骨组织衰老。这种将NIR响应纳米医学与外泌体介导的细胞间通讯相结合的双功能系统为开发先进的骨修复材料提供了一种新策略,可能通过协同机械增强和生物激活机制来解决目前骨科应用中的局限性。