Faecal Microbiota Microsphere Contributed to Relieving Gut Barrier Damage in Colitis

Gut microbiota play an important role in many diseases. However, few researches evaluated the effect of faecal bacteria transplantation in protecting gut barrier during colitis. In this study, we focused on the effect of faecal microbiota encapsulated alginate microsphere on dextran sulfate sodium (DSS) induced colitis mice. Bacteria are sensitive to gastric juice and digestive enzymes, which is the dilemma of utilizing microbiota. Through taking advantage of alginate, microspheres that could protect its contents were fabricated. In vitro drug release experiment demonstrated their ability in protecting contents in simulated gastric fluid and release contents in simulated intestinal fluid. Thus, the resultant microsphere could be used in faecal microbiota transplantation. In vivo , the transplantation of faecal microbiota from healthy mice showed protection against DSS induced oxidative stress and gut barrier damage. Our findings suggest that the faecal microbiota transplantation could be a potential effective treatment for protecting gut barrier in inflammatory bowel diseases (IBD) colitis with faecal microbiota encapsulated alginate microsphere.