Label-Free Fluorescent Aptasensor for Ochratoxin—A Detection Based on CdTe Quantum Dots and (N-Methyl-4-pyridyl) Porphyrin

With the widespread contamination of ochratoxin A (OTA), it is of significant importance for detecting OTA in foods and traditional Chinese medicine (TCM). In this study, a novel label-free fluorescent aptasensor utilizing the interaction between OTA-triggered antiparallel G-quadruplex and (N-methyl-4-pyridy) porphyrin (TMPyP) for the rapid and sensitive determination of OTA was established. The fluorescence of CdTe quantum dots (QDs) could be quenched by TMPyP. In the presence of analyte (OTA), the aptamer could recognize OTA and transform from a random coil to the antiparallel G-quadruplex. The interaction between G-quadruplex and TMPyP could release CdTe QDs from TMPyP, and thus recover the fluorescence of CdTe QDs. Under optimized conditions, the detection limit of the designed aptasensor was 0.16 ng mL−1, with a linear range of 0.2 to 20 ng mL−1. Furthermore, this aptasensor showed high selectivity toward OTA against other structural analogs and other mycotoxins, and was successfully applied inAstragalus membranaceussamples. The presented aptasensor for OTA detection could be a promising tool for the field monitoring of food and TCM.Keywords:ochratoxin A;porphyrin;quantum dots;G-quadruplex aptamerKey Contribution:A label-free fluorescent aptasensor for the sensitive determination of ochratoxin A utilizing the interaction between OTA-triggered G-quadruplex and TMPyP is reported.