Graphene quantum dots-based electrochemiluminescence detection of DNA using multiple cycling amplification strategy

In this study, a novel strategy for amplified electrochemiluminescence (ECL) detection of DNA was proposed based on excellent ECL activity of graphene quantum dots (GQDs) coupled with multiple cycling amplification technique. A new type of graphene QDs with well ECL property and uniform size were firstly synthesized, then the graphene QDs were assembled on the electrode by poly (diallyldimethylammonium chloride) (PDDA)-graphene oxide (GO) nanocomposites, which could greatly improve ECL signal and stability of QDs. A novel signal-on ECL biosensor for DNA analysis was designed by using ECL quenching of gold nanoparticles (NPs) to graphene QDs combined with endonuclease–aided cyclic amplification strategy. As a result, the proposed strategy can be conveniently expanded to other biosensing application, especially in clinical diagnoses.