Structural features and anti-gastric cancer activity of polysaccharides from stem, root, leaf and flower of cultivated Dendrobium huoshanense

The structure features and anti-gastric cancer activities in vitro of stem, root, leaf and flower polysaccharides from cultivated Dendrobium huoshanense were investigated systematically. Stem polysaccharide (cDHPS) was composed of →4)-β-D-Glc p -(1→, →4)-β-D-Man p -(1→, →4)-3- O -acetyl-β-D-Man p -(1→ with the molecular weight of 2.59 × 10 5  Da; root polysaccharide (cDHPR) was composed of →3,5)-α-L-Ara f -(1→, →4)-β-D-Glc p -(1→, →4)-β-D-Man p -(1→, →4,6)-β-D-Man p -(1→, →6)-α-D-Gal p -(1→ and terminal β-L-Ara f with the molecular weight of 1.41 × 10 4  Da; leaf polysaccharide (cDHPL) was composed of →4)-β-D-Glc p -(1→, →4)-β-D-Man p -(1→, →4)-3- O -acetyl-β-D-Man p -(1→, →3,6)-β-D-Man p -(1→ and terminal α-D-Gal p with the molecular weight of 2.09 × 10 5  Da; and flower polysaccharide (cDHPF) was composed of →4)-β-D-Glc p -(1→, →4)-β-D-Man p -(1→, →3,6)-β-D-Man p -(1→ and terminal α-D-Gal p with the molecular weight of 4.78 × 10 5  Da. Among these four polysaccharides, cDHPS showed the best anti-gastric cancer activity evidenced by the inhibited growth and c-myc expression as well as the enhanced apoptosis and p 53 expression of murine forestomach carcinoma (MFC) cells, suggesting their difference in anti-gastric cancer activity should be contributed to their difference in structure features.