Response of extracellular polymeric substances and microbial community structures on resistance genes expression in wastewater treatment containing copper oxide nanoparticles and humic acid

The extracellular polymeric substances (EPS) and microbial community structures were investigated in wastewater treatment containing copper oxide nanoparticles (CuO NPs) (reactor R1) and CuO NPs and humic acid (HA) (reactor R2) using both sequencing batch bioreactors (SBRs), and their response on resistance genes expression was analyzed. The removal of influent chemical oxygen demands (COD) and NH 4 + -N was moderately influenced under CuO NPs (5 mg/L) stress, while the function of HA (10 mg/L) was not reflected. However, the EPS production and microbial community were affected by the HA addition. The expression of different antibiotic resistance genes (ARGs), metal-resistance genes (MRGs), and int I1 was related to the primary compositions of polysaccharides and proteins in EPS and different microbial communities at the genus level. Furthermore, the expression of resistance genes was not stimulated under CuO NPs stress, and supplying HA was suggested to reduce their expression in wastewater treatment.