Design and investigation of salecan/chitosan hydrogel formulations with improved antibacterial performance and 3D cell culture function

Hydrogel formulations are considered to be promising dosage forms for clinical delivery of antibiotics but suffer from many limitations such as polymers selection and crosslinking methods. Herein, dynamic Schiff base reaction crosslinked hydrogels (denoted as CO) with ‘switchable’ channels were designed based on Chitosan and Salecan. Chitosan maintains the pH sensitive property while providing functional groups for the reversible bond formation. Equilibrium water content, swelling and degradation behavior, dynamic rheology and cytotoxicity assay were investigated to characterize the hydrogels’ properties. By simulating and comparing four classic models, the drug release kinetics and mechanism were studied in detail. HeLa cells were encapsulated to further exploring the feasibility of 3D cell culture. All the results revealed CO hydrogels possessed excellent biological functions to maintain cell growth. Therefore, CO hydrogels can be served as a promising carrier for drug delivery and also expected to apply in many other medical fields.