Spherical nanoparticle-modified bacterial cellulose drives SH−SY5Y cell differentiation and inhibits bacterial proliferation

Bacterial cellulose (BC) possesses good physical and mechanical properties for tissue engineering, but its negatively charged surface hinders cell adhesion . Here, the surface charge of BC was modulated with 2 −bromoethylamine hydrobromide (2 −BrEA) via a one-step synthesis method performed in alkaline aqueous solutions at room temperature. Positively charged spherical nanoparticles were thus formed in situ on BC. The amination degree of ethylamine-modified BC materials (BC−EAs) reached 2.80 mmol/g; whereas the crystallinity, fiber diameter, and water contact angle were 55.5 − 65.5%, 30 − 45 nm, and 32.5 − 44.9°, respectively. BC−EAs possess good antibacterial effectiveness against Escherichia coli and Staphylococcus aureus . The CCK− 8 and live/dead assays suggest that BC modified with 0.09 g/mL 2 −BrEA (BC−EA3) is biocompatible and possesses a high efficiency for promoting human neuroblastoma SH−SY5Y cell proliferation and differentiation. Its antibacterial ability is beneficial for preventing the formation of biofilms in the extracellular matrix during tissue and organoid formation, while the properties promoting SH−SY5Y cell proliferation and differentiation make BC−EA3 a good candidate for nerve- or other tissue engineering.