Pre-protective effect of lipoic acid on injury induced by H2O2 in IPEC-J2 cells

A lpha-lipoic acid (LA) is considered to be a universal antioxidant. The intestine is very sensitive to a wide range of stressors for it exposed in a complex microenvironment that usually contained excess free radicals. The objective of this study was to evaluate the pre-protective effect of LA on intestinal epithelial cell injury induced by H 2 O 2 . In this research, IPEC-J2 cells were divided into three groups: the control group, the H 2 O 2 -treated group, and the LA pre-protective group. Cell viability, intracellular ROS, DNA damage integrity, and antioxidant enzyme activity of all groups were measured. Quantitative reverse transcription-PCR was used to determine the influence of oxidative stress and LA on the GH/IGF-1 axis in IPEC-J2 cells. The research selected 1 mM H 2 O 2 and 10 μg/ml as modeling concentration. The data indicated that H 2 O 2 can significantly increase the intracellular free radicals, leading to serious DNA damage and significantly reduce superoxide dismutase, glutathione peroxidase, catalase, and lipase activity. LA-protected IPEC-J2 cells against H 2 O 2 -induced injury by scavenging hydroxyl radical. In the current study, LA pre-protective group share a lower intracellular ROS and lower DNA damage compare to H 2 O 2 -treated group ( P  < 0.01); and also, a higher lipase activity were detected in LA pre-protective group compare to H 2 O 2 -treated group ( P  < 0.05). But the mechanism between oxidative stress, LA effect, and GH/IGF-1 axis is still unknown. The model used in the current study is also suitable for other feed additive screening.