In Vitro Assessment of Biodegradable and Biocompatible Brain-Derived Neurotrophic Factor-Mesenchymal Stem Cells Incorporated Chitosan/Gelatin Scaffold

In this paper, the biocompatible chitosan/gelatin scaffolds at different chitosan/gelatin ratios were fabricated by a facile method. The important physical parameters of the scaffolds, including density, porosity and tensile strength were measured. MSCs were transfected to introduce brain-derived neurotrophic factor (BDNF) gene into the cells. To evaluate the bioactivity of the scaffolds, the gene-modified BDNF-MSCs were cultivated on the scaffolds, and the cell proliferation and BDNF expression level were determined.