Based ‘successive’ nucleophilic substitution mitochondrial-targeted H2S red light emissive fluorescent probe and its imaging in mice

The research of endogenous hydrogen sulfide (H 2 S) biology function, pathological and pharmacological effects has been active in the field of chemists and biologists in recent years. Especially, consequent H 2 S detection mechanisms and biological applications based on fluorescence probe emerged in endlessly, competing ideas, each had its advantage. In facts, the reaction mechanism and luminescence properties play a decisive role in the biology application of H 2 S fluorescence probe. In this despite booming season, in the process of research we still skillfully conceived, in view of H 2 S with small structure being able to do successive nucleophilic substitution in the same molecules, high selective H 2 S subcellular detection was realized. Specifically, we take the cyanine (Cy7) with positive charge as a red fluorophore, through the introduction of disulfide bond and carbonate to build the specific reaction sites of H 2 S, thus avoiding the interference of other sulfur-containing substances in biological systems. After reaction with H 2 S, the Probe released red fluorophore, realizing mitochondria target H 2 S detection. Because the advantages of red fluorescence emission probe, the Probe successfully was applied imaging in mice.