A new “turn-on” fluorescence probe based on hydrazine-triggered tandem reaction

Hydrazine is extremely harmful to the human body. The leakage of hydrazine is liable to cause potential safety hazards. Here, we reported a new fluorescence probe based on the tandem reaction . The hydrazine-triggered hydrazinolysis-cyclization resulted in the formation of the iminocoumarin. The fluorescence intensity at 522 nm of the probe increased after the reaction with hydrazine. There was a linear relationship between the fluorescence intensity and the concentration of hydrazine (0.14–120.00 μM). The LOD of the probe to N 2 H 4 was 1.36 ppb. Notably, the probe could detect hydrazine in BT474 cells and tap water.