Fabrication of homogeneous waffle-like silver composite substrate for Raman determination of trace chloramphenicol

Waffle-like anodized aluminum oxide homogeneously immobilized with Ag nanoparticles (AAO/Ag) is rationally designed and fabricated as surface-enhanced Raman scattering (SERS) substrate. The as-prepared SERS substrate is characterized with transmission electron microscope (TEM), scanning electron microscopy (SEM), UV–Vis spectrophotometer, and Fourier transform infrared spectrometer (FT-IR). The AAO/Ag substrate shows good uniformity of the Raman signals (RSD = 7.02%) due to waffle-like AAO supporting the well-dispersed Ag nanoparticles. For real application, the AAO/Ag substrate is used for rapid determination of chloramphenicol (CAP) in honey with low detection limit (4.0 × 10 −9  mol L −1 ) and good linearity from 1.0 × 10 −5 to 1.0 × 10 −8  mol L −1 based on the SERS peak at 1348 cm −1 . The better accumulation in the short pore path of AAO improves the target molecule approaching into the vicinity of hot spots of Ag nanoparticles. The high selectivity for CAP is attributed to the strong interaction between -NO 2 group in CAP and the composite substrate. Schematic representation of the preparation of SERS substrate, AAO 150 /Ag 10 -5 composite nanoparticles, and antibiotic determination.